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| Morvan, Annie. |
| Des anticorps monoclonaux spécifiques de l'hémolymphe de Crassostrea gigas (Mollusques : Bivalves) ont été caractérisés en immunohistologie. Plusieurs sous- populations hémocytaires ont été identifiées sur la base de leur antigénicité, et certaines d'entre elles ont été séparées par centrifugation isopycnique en gradient de Percoll. Cette technique a été comparée à une méthode d'immunopurification associant l'anticorps monoclonal HCG 12 et des billes magnétiques. L'activité de chimioluminescence résultant de la phagocytose de particules de zymosan a été estimée pour des sous- populations d'hémocytes. Le phénotype antigénique HCG 12 semble étroitement corré lé avec l'activité de chimioluminescence. Parmi les anticorps monoclonaux spécifiques d'épitopes... |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00429/54051/55344.pdf |
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| Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric. |
| The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few... |
| Tipo: Text |
Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF |
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